ABSTRACT The objective with this study was to evaluate the libido and sexual behavior of Pantaneiro stallions in Brazil in two periods, the rainy and dry seasons. Their sexual behavior was evaluated during mating with mares in estrus, and the libido was scored for statistical analysis. The reaction time and time to mount and ejaculate were 78.1±64.6 s and 289.0 s, respectively. The most relevant events of sexual behavior observed in the Pantaneiro stallions were mounting without erection, mounting without ejaculation, smelling, and vocalizing. In general, the season did not affect the libido of stallions. Even with the high temperatures and humidity, which could cause thermal stress, the stallions showed good libido score during most of evaluations, with some individual differences. Pantaneiro stallions are able to breed mares during the entire year and could facilitate the breeding management to raise the number of Pantaneiro horses of the herds and optimize the use of the stallions during the breeding season, with more females per male.

Both the study of Brazilian wild mammal fauna and the conditions that foster the preservation of endangered species, such as Brazilian Maned-wolf (Chrysocyon brachyurus), in wild life are of extreme importance. In order to study the resistance profile of microbiota bacterial colonizing Brazilian Maned-wolf, this work investigated samples from eight male captive and free roaming animals originating from different Brazilian geographical regions. Samples for microbiological purposes were collected with swabs and kept in appropriate transport medium. Using routine microbiological techniques, the isolated bacteria were tested toward antimicrobial drugs by the agar disk diffusion method. Results showed that all samples from wild animals were sensitive toward all drugs tested. Conversely, the resistance profile of bacteria isolated from captive animals varied among strains and animal body site location. Escherichia coli samples from prepuce, anus and ear showed multi-resistance toward at least four drugs, especially against erythromycin and tetracycline, followed by Proteus mirabilis and P. vulgaris strains isolated from anus and ear. Among Gram-positive bacteria, strains of coagulase-negative staphylococci showed multi-resistance mainly toward erythromycin and amoxicillin. The work discusses these findings and suggests that profile of multi-resistance bacteria from captive subjects may be attributed to direct contact with human or through lifestyle factors such as feeding, predation or contact of animals with urban animals such as birds, rodents, and insects from surrounding environments.

Objetivou-se neste trabalho verificar o efeito da adição de alanina, glicina e glutamina ao meio crioprotetor seminal de garanhões Mangalarga Marchador. Foram utilizados cinco garanhões provenientes do haras Lugavi. Três ejaculados de cada garanhão, após coleta e avaliação, foram divididos em seis tratamentos: sem adição de aminoácidos, adição de 40 mM de alanina, adição de 40 mM de glicina, adição de 60 mM de glutamina, adição de 7 mM de alanina + 7 mM de glicina + 20 mM de glutamina e adição de 40 mM de alanina + 40 mM de glicina + 60 mM de glutamina ao diluente de congelamento convencional. Foram avaliados os parâmetros motilidade total, motilidade progressiva, retidão, linearidade, velocidade média do percurso, velocidade linear, velocidade curvilínea, amplitude lateral de cabeça e frequência de batimentos dos espermatozoides por meio de análise espermática assistida por computador (CASA), funcionalidade da membrana plasmática por choque hiposmótico e integridade de membrana acrossomal pelo teste FITC-PSA. Não houve melhora nos parâmetros de motilidade e cinética espermática nem na funcionalidade de membrana, entretanto, a adição de aminoácidos ao meio crioprotetor seminal aumentou o número de espermatozoides com membrana acrossomal íntegra. Os melhores resultados neste teste são obtidos com 40 mM de alanina (78,6 ± 13,6), 40 mM de glicina (74,7 ± 19,6) ou 7 mM de alanina + 7 mM de glicina + 20 mM de glutamina (76,8 ± 17,5), porém outros estudos devem ser realizados para determinação da concentração ideal desses componentes.

The objective of this study was to analyze the effect of the addition of alanine, glycine and glutamine on frozen seminal extender of Mangalarga marchador breed stallions. Five stallions were used from the Lugavi stables. Three ejaculates from each stallion were divided into six treatments: without the addition of amino acids, addition of 40 mM alanine, addition of 40 mM glycine, addition of 60 mM glutamine, addition of 7 mM glycine, alanine 7 mM + 20 mM glutamine and addition of 40 mM of glycine + 40 mM of alanine + 60 mM of glutamine to conventional frozen extender. The parameters evaluated were total motility, progressive motility, straightness, linearity, path velocity, progressive velocity, track speed, lateral amplitude and beat frequency by computer assisted sperm analysis (CASA), functionality of plasmatic membrane by hypo-osmotic shock and acrossomal membrane integrity by the FITC-PSA test. No improvement was found in the kinematic parameters and sperm motility and nor in membrane functionality but the addition of amino acids to seminal extender resulted in a higher integrity of the acrossomal membrane. The best results obtained in this test are with 40 mM alanine (78.6 ± 13.6), 40 mM glycine (74.7 ± 19.6) or 7 mM glycine, alanine 7 mM + 20 mM glutamine (76.8 ± 17.5), however, more studies should be conducted to reach the ideal concentration of these components.